Huang Zhuo

Dr Zhuo Huang obtained a pharmacy degree in School of Pharmaceutical Science from Beijing University, followed by a PhD with Prof. Alasdair Gibb at the Department of Pharmacology, University College London during which time he carried out work on Mg2+ block properties of triheteromeric (GluN1-GluN2B-GluN2D) NMDA receptors on dopaminergic neurons. He subsequently worked in Dr Mala Shah’s laboratory at UCL School of Pharmacy where he started his work on understanding ion channel properties and function in entorhinal cortical dendrites under physiologic and epileptic conditions. In 2013, he was awarded “Hundreds of talents” Program of Peking University and returned to the Department of Molecular and Cellular Pharmacology, Beijing University as a senior investigator.

Address:

Department of Molecular and Cellular Pharmacology, State Key Laboratory of Nature and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xue Yuan Road, HaiDian District, Beijing, P.R.China, 100191

Email：huangz@hsc.pku.edu.cn

 

 Research Interest

Research in my laboratory is primarily directed towards understanding the cellular and molecular mechanisms of temporal lobe epilepsy and Parkinson’s disease. We are focusing our attention on the function of voltage-gated ion channels which play important role on neuronal excitability. The alteration in expression, subcellular localization or biophysics of these channels leads to abnormal excitability of single neuron and neuronal network, thus facilitate the development of the disease.

 

Specific projects include

Cortical calcium channel and temporal lobe epilepsy

The regulatory mechanism of HCN (Hyperpolarization-activated cyclic nucleotide gated ) channels on midbrain dopamine neurons in Parkinson’s disease

 

Publications

Peer-reviewed articles:

1.         Liu YQ, Lai SR, Ma WN, Ke W, Zhang C, Liu SM, Zhang Y, Pei F, Li SY, Yi M, Shu YS, Shang YF, Liang J, and Huang Z (2017) CDYL suppresses epileptogenesis through repression of axonal Nav1.6 sodium channel expression. Nature Communication. Aug 25;8(1):355. doi: 10.1038/s41467-017-00368

2.         Huang Z,Lujan R, Kadurin I, Uebele VN, Renger JJ, Dolphin AC, Shah MM. (2011) Presynaptic HCN1 channels regulate Ca(V)3.2 activity and neurotransmission at select cortical synapses, Nature Neuroscience. Apr;14(4):478-86

3.         Huang Z, Lujan R, Martinez-Hernandez J, Lewis AS, Chetkovich DM, Shah MM. (2012) TRIP8b-Independent Trafficking and Plasticity of Adult Cortical Presynaptic HCN1 Channels. J Neurosci. Oct 17;32(42):14835-48.

4.         Huang Z, Walker MC, Shah MM. (2009) Loss of dendritic HCN1 subunits enhances cortical excitability and epileptogenesis, J. Neurosci., Sep 2; 29(35):10979-88.

5.         Martinello K, Huang Z, Lujan R, Tran B, Watanabe M, Cooper EC, Brown DA, Shah MM.(2015) Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells, Neuron, 85, 346-363

6.         Li X, Liu D, Ma Y, Du X, Jing J, Wang L, Xie B, Sun D, Sun S, Jin X, Zhang X, Zhao T, Guan J, Yi Z, Lai W, Zheng P, Huang Z, Chang Y, Chai Z, Xu J, Deng H.（2017）Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State. Cell Stem cell. Jun 20. pii: S1934-5909(17) 30185-6.

7.         Huang Z, Li G, Aguado C, Lujan R, Shah MM. (2016) HCN1 channels reduce the rate of exocytosis from a subset of cortical synaptic terminals. Scientific ReportsJan 10;7:40257.

8.         Huang Z & GibbA.J. (2014) Mg²⁺ block properties ofNMDA receptors in neonatal rat substantia nigra pars compacta dopaminergic neurones. J Physiol.  15; 592(10):2059-78.

9.         Shah MM, Huang Z, Martinello K. (2013) HCN and K(V)7 (M-) channels as targets for epilepsy treatment. Neuropharmacology.  Jun; 69:75-81.

10.     Zhou J, Tang Y, Zheng Q, Li M, Yuan T, Chen L, Huang Z, Wang K.  (2015) Different KChIPs compete for heteromultimeric assembly with pore-forming Kv4 subunits. Biophys J. 108(11):2658-69. 

11.     Ren LB, Wang XY, Xie Q, Liu JX, Huang Z, Du Ying, Wang JY (2007) Study on correlation of contents of light rare earth elements in rats’brains, Chinese Journal Of Analysis Laboratory,26, 108-111)

12.     Ren LB, Wang XY, Xie Q, Liu JX, Huang Z, Du Ying, Wang JY (2007) Study on correlation of contents of light rare earth elements in rats’brains, Chinese Journal Of Analysis Laboratory, 26, 108-111)

13.     Liu JX, Wang XY, Xie Q, Liu Y, Zhang JH, Huang Z, Ren LB, Du Y, Wang JY (2007) Study on the characteristics of content and distribution of light rare earth elements in rat testis, Journal of Hygiene Research, 06,734-736 )

14.     Wang JY, Ouyang L, Liu YQ, Xie Q, Huang Z, Tu P F, Guo XL, Liu HS (2004), Preliminary attempt at the speciation of 25-elements in the chinese medicinal herb, China Journal Of Chinese Materia Medica,29, 753-759

15.     Wang JY, Zhu HD, Ouyang L, Liu YQ, Wang XY, Huang Z, Wang NF, Liu HS (2004), Determination of trace Cs, Th and U in ten kinds of human autopsy tissues by ICP-MS, Specroscopy and Spectral Analysis, 24, 1117-1120

16.     Huang Z., Wang Q., Wang JY (2003) RP-HPLC Method for Determination of Propofol in Human Serum, The Chinese Journal of Clinical Pharmacology, 19, 293-296

 

Books:

 

Signal Processing in Neuroscience, Springer, 2016

 

Patent：

 

Invited seminars and talks:

1.        Pre-synaptic HCN channels and excitatory synaptic transmission in the entorhinal cortex, Invited talk at Physoc. Annual Meeting in Manchester, UK, June 2010

2.        CDYL controls neuron excitability by regulating the transcription of Nav1.6 sodium channels, Invited talk at Sino-Israel Workshop of Neuroscience: Ion channel, Synapse and Neuro-issues,Shanghai, China, April 2015

3.        The epigenetic factor CDYL inhibits intrinsic neuronal excitability and suppresses epileptogenesis through repression of axonal Nav1.6 sodium channel expression, Invited talk at 6^th International Conference for ion channels, QinDao China, June 2017

4.        Status epilepticus induced downregulation of ERG3 potassium channels facilitates the development of epilepsy in mouse temporal lobe epilepsy model, Invited talk at 25^th International symposium on Morphological sciences, Xi`an China, July 2017

 

Poster Presentations:

1.        Zhang J, Huang Z. (2016) Blockade of HCN channels in nucleus accumbens cholinergic neurons prevents cue-induced relapse of cocaine-seeking, The seventeenth National Academic Seminar of Neuropsychopharmacology, Hefei, China 2016

2.        XueqinJ, Zhuo H (2017) Dopamine D2 receptors Induce Functional Plasticity by Modulation of Axonal Cav3.2 Calcium Channels in Stellate Cells of Entorhinal Cortex, The 6^th International Ion Channel Conference, Qing Dao, China, 2017.

3.        XueqinJ, Zhuo H. (2016)Dopmaine D2 receptors Modulate Spatial Cognition via Axonal T-type Calcium Channels in Medial Entorhinal Cortex,The seventeenth National Academic Seminar of Neuropsychopharmacology, Hefei, China, 2016.

4.        Shirong L, Zhuo H (2017) The Epigenetic Factor CDYL Inhibits Intrinsic Neuronal Excitability and Suppresses Epileptoge n e s is through Repression of Axonal Nav1.6 Sodium Channel Expression, The 6^th International Ion Channel Conference, Qing Dao, China, 2017.

5.        Shirong L, Zhuo H (2016) The Epigenetic Factor CDYL Inhibits Intrinsic Neuronal Excitability and Suppresses Epileptoge n e s is through Repression of Axonal Nav1.6 Sodium Channel Expression, The seventeenth National Academic Seminar of Neuropsychopharmacology, Hefei, China, 2016.

6.         Kuo Xiao, Zhiming Sun, Minghua Fan, Jingliang Zhang, Xueqin Jin, Zhuo Huang (2017)Reduction in functional ERG3-mediated currents facilitates the progression of temporal lobe epilepsy in mice”The 6^th International Ion Channel Conference, Qing Dao, China, 2017.

7.        Liu YQ, Liang J, Huang Z. (2017) Epigenetic factor CDYL suppresses epileptogenesis through repression of axonal Nav1.6 channel expression, The 6th International Ion Channel Conference,Qing Dao, China 2017

8.        Huang Z, Lujan R, Shah MM. (2010) Pre-synaptic HCN channels and excitatory synaptic transmission in the entorhinal cortex, SfN abstract

9.        Huang Z, Lujan R, Shah MM. (2010) Pre-synaptic HCN channels and excitatory synaptic transmission in the entorhinal cortex, Journal of Physiology Proc Physiol Soc.

10.    Huang Z, Lujan R, Shah MM. (2009) Effects of presynaptic HCN channels on entorhinal cortical glutamatergic synaptic transmission: implications for epilepsy, BNA abstract

11.    Huang Z, Shah MM. (2008)Role of HCN1 subunits in Entorhinal Cortical neuronal excitability and epilepsy,Harden Conference abstract

12.    Huang Z, Gibb AJ. (2008) Competition between memantine and magnesium for block of NMDA receptor channels in dopaminergic neurons of neonatal rat substantia nigra, Journal of Physiology Proc Physiol Soc

13.    Huang Z, Brothwell S, Jones S and Gibb AJ. (2007) Deactivation kinetics of NMDA receptors in rat substantia nigra dopaminergic neurons, SfN abstract

14.    Huang Z, Gibb AJ. (2007) Functional NMDA receptor on STN.Journal of Physiology Proc Physiol Soc, PC398

15.    Huang Z, Gibb AJ. (2006) Magnesium block of NMDA receptors in dopaminergic neurons of neonatal rat substantia nigra pars compacta. Journal of Physiology Proc Physiol Soc , 3, PC157

 

Honors and Awards

1.        UK Physiological Society Travel Award, UCL, 2010

2.        UCL postgraduate Travel Award for SfN, 2007

3.        UCL Old Student Association Scholarship, 2006-2007

4.        UK Overseas Research Student Scholarship, 2005

5.        Peking university scholarships by the International Health Exchange Center, 1999-2002